Detection of a genetic mutation for myotonia congenita among Miniature Schnauzers and identification of a common carrier ancestor     

Bhalerao DP  Rajpurohit Y  Vite CH  Giger U 《American journal of veterinary research》2002,63(10):1443-1447

OBJECTIVE: To develop a molecular genetic test to detect the mutant skeletal muscle chloride channel (CIC-1) allele that causes myotonia congenita in Miniature Schnauzers and to analyze the relationship of affected and carrier dogs. ANIMALS: 372 Miniature Schnauzers from the United States, Canada, Australia, and Europe that were tested between March 2000 and October 2001. PROCEDURE: The sequence surrounding the mutation in the CIC-1 allele was amplified by use of a unique pair of primers. Polymerase chain reaction (PCR) products were digested with the restriction enzyme Hpy CH4 III and separated on a 6% polyacrylamide gel. Pedigrees from all available carrier and affected dogs were analyzed, and a composite pedigree was established. RESULTS: Enzyme digestion of PCR products of the normal CIC-1 allele resulted in 3 fragments of 175, 135, and 30 bp, whereas PCR products of the mutant allele resulted in fragments of only 175 and 165 bp. Of the 372 Miniature Schnauzers, 292 (78.5%) were normal, 76 (20.4%) were carriers, and 4 (1.1%) were affected (myotonic) dogs. Frequency of the mutant allele was 0.113. Pedigree analysis revealed that a popular sire, documented to be a carrier, was a common ancestor of all carriers and affected dogs. CONCLUSIONS AND CLINICAL RELEVANCE: A PCR-based enzyme digestion DNA test was developed. The mutant allele for this disease is frequent in Miniature Schnauzers that are related to a common carrier ancestor. Breeding dogs should be tested by this specific DNA test to help limit the spread of this deleterious mutation.  相似文献   

Results of cerebrospinal fluid analysis, neurologic examination findings, and age at the onset of seizures as predictors for results of magnetic resonance imaging of the brain in dogs examined because of seizures: 115 cases (1992-2000)     

Bush WW  Barr CS  Darrin EW  Shofer FS  Vite CH  Steinberg SA 《Journal of the American Veterinary Medical Association》2002,220(6):781-784

OBJECTIVE: To determine whether neurologic examination findings, results of CSF analysis, or age at the onset of seizures could be used to predict whether results of magnetic resonance imaging (MRI) would be normal or abnormal in dogs with seizures. DESIGN: Retrospective study. ANIMALS: 115 dogs. PROCEDURE: Information on results of neurologic examination, results of CSF analysis, age at the onset of seizures, and results of MRI was obtained from the medical records. RESULTS: Results of MRI were abnormal in 61 dogs and normal in 54. Sensitivity and specificity of neurologic examination alone were 77 (47/61) and 91% (49/54), respectively. Sensitivity and specificity of CSF analysis alone were 79 (48/61) and 69% (37/54), respectively. Results of MRI were abnormal for 12 of 28 (43%) dogs with abnormal CSF analysis results and normal neurologic examination results but for only 2 of 35 (6%) dogs with normal CSF analysis and normal neurologic examination results. Similarly, results of MRI were abnormal for 36 of 37 (97%) dogs with abnormal CSF analysis and abnormal neurologic examination results but for only 11 of 15 (73%) dogs with normal CSF analysis results and abnormal neurologic examination results. Age at the onset of seizures (< 6 vs > or = 6 years old) was not significantly associated with results of MRI. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that neurologic examination findings and results of CSF analysis are useful in predicting whether results of MRI will be abnormal in dogs examined because of seizures, but age at the onset of seizures is not.  相似文献   

Association of cauda equina compression on magnetic resonance images and clinical signs in dogs with degenerative lumbosacral stenosis     

Mayhew PD  Kapatkin AS  Wortman JA  Vite CH 《Journal of the American Animal Hospital Association》2002,38(6):555-562

Magnetic resonance imaging (MRI) was used to examine the lumbosacral spine of 27 dogs with degenerative lumbosacral stenosis. Four normal dogs were also similarly imaged. Compression of the soft-tissue structures within the vertebral canal at the lumbosacral space was assessed in two ways: by measuring dorsoventral diameter on T1-weighted sagittal images and cross-sectional area on transverse images. The severity of the clinical signs was compared to the severity of cauda equina compression. No significant correlation was found. It is concluded that degree of compression as determined by MRI at time of presentation is independent of disease severity.  相似文献   

Suspected Albuterol Intoxication In A Dog     

Charles H. Vite DVM  Roger W. Gfeller DVM 《Journal of Veterinary Emergency and Critical Care》1994,4(1):7-13

A suspected case of intoxication of a dog by the beta₂-adrenergic agonist albuterol is discussed. Clinical abnormalities observed included hypokalemia, weakness, tachycardia, tachypnea, hyperthermia, and delirium. Treatment with high-dose potassium supplementation helped to resolve these abnormalities.
Adrenergic control of potassium homeostasis is beta₂-mediated and causes the intracellular uptake of potassium, primarily in liver and muscle. The mechanism of action of albuterol in decreasing serum potassium, its clinical applications in humans, and possible application in dogs are discussed.  相似文献   

Clinical peripheral neuropathy associated with diabetes mellitus in 3 dogs     

Morgan MJ  Vite CH  Radhakrishnan A  Hess RS 《The Canadian veterinary journal. La revue veterinaire canadienne》2008,49(6):583-586

Clinical and electrodiagnostic findings in 3 spontaneously diabetic dogs with clinical peripheral neuropathy (PN) are reported. Clinical signs of a PN may develop in diabetic dogs with adequate glycemic control. In addition, laryngeal paralysis may develop in association with diabetes mellitus in dogs with clinical PN.  相似文献   

CT-guided stereotactic brain biopsy using the Kopf stereotactic system     

Mark T Troxel  Charles H Vite 《Veterinary radiology & ultrasound》2008,49(5):438-443

This report describes computed tomography (CT)-guided stereotactic brain biopsy using the Kopf stereotactic system, a commercially available patient restraint system which does not require additional modification for use in small animals. The accuracy of biopsy needle placement was determined by injecting dilute iohexol into cadaver brains and comparing the three-dimensional coordinates of the desired target location to the actual needle tract observed on postcontrast CT images. Overall mean error in needle placement in a dorsoventral trajectory was 0.9 +/- 0.9 mm (n = 80 injections) for dogs and 1.0 +/- 1.1 mm (n = 30 injections) for cats. The overall mean error in needle placement via an oblique trajectory in five dogs was 1.7 +/- 1.6 mm (n = 12 injections). These results suggest that this system can be used to successfully place a biopsy needle into the brain to obtain biopsies from small lesions.  相似文献   

短尾鮠精子细胞和精子的超微结构研究     

张汉峰  魏刚  黄林  代大临  陈永强  张运明 《西南大学学报(自然科学版)》2005,27(5):696-699

用透射电镜观察了短尾鮠精子细胞和精子的形态以及细胞核、拟染色体和线粒体等的结构变化规律。结果表明,随着精子细胞的发育,核质凝聚程度逐渐增强。精子细胞时期细胞器较丰富,到精子细胞后期,细胞器的形态和数量发生了较大变化。短尾鮠的精子具有椭圆的头部和复杂的中片;近侧中心粒和远侧中心粒靠近核的中央;鞭毛呈9+2轴丝结构,并具有由外膜折迭形成的波浪形的鳍状结构。  相似文献   

Effects of L-carnitine administration on treadmill test performance of untrained dogs     

CH. Iben 《Journal of animal physiology and animal nutrition》1999,82(2-3):66-79

Introduction   Carnitine (trimethyl-γ-amino-β-hydroxybutyrate; molecular weight 161 g) is not only essential for the transport and utilization of long-chain fatty acids, it also occurs as a medium and short-chain ester and serves as an acetyl and acyl pool. Short-term muscle exercise causes an increase of acetyl-carnitine levels in the serum and in the liver. It prevents the accumulation of acyl-coenzyme A (CoA) and provides the organism with CoA by producing acetyl-carnitine from acetyl-CoA. Human muscle tissue contains approximately 10 μmol CoA/kg, whereas carnitine levels range between 2000 and 5000 μmol/kg.  相似文献